Flat Assembler is a fast and efficient self-assembling 80x86 assembler. It supports x86 and x86-64 instruction sets with MMX, 3DNow!, SSE up to SSE4, AVX, AVX2, and XOP extensions. It can produce output in binary, MZ, PE, COFF, or ELF format. It includes powerful but easy-to-use macroinstruction support and does multiple passes to optimize the instruction codes for size. It is written entirely in assembly language.
asfpga is an assembler written for use in FPGA design. It can be easily modified for a particular instruction set. The ultimate goal of this software is to allow a FPGA designer to easily write assembly code for a custom instruction set. The current version allows you to create a listing file, a memory dump file which can be used in debugging HDL code using $readmemh() or equivalent routine, and a binary file which can be used to program an EPROM.
Yasm is a complete rewrite of the NASM assembler. It currently supports the x86 and AMD64 instruction sets, accepts NASM and GAS assembler syntaxes, outputs binary, ELF32, ELF64, COFF, Mach-O (32 and 64), RDOFF2, Win32, and Win64 object formats, and generates source debugging information in STABS, DWARF 2, and CodeView 8 formats.
h2incn tries to directly convert C/C++ headers to Nasm-style include files, and can be used in a makefile. It is useful if you want to use the same structures or external variable declarations in C and assembler code, and you don't want to use two separate files and update both each time you change something. It is written in a mix of C and C++ code. It currently works for simple files.