All releases of PerlPrimer


Release Notes: This release again restores compatibility with the Ensembl online database.


Release Notes: This release fixes compatibility with the Ensembl database, and enables the retrieval of unannotated genes from Ensembl (using the Ensembl Gene ID). Compatibility with NCBI BLAST search results was restored. There's also an update to the ORF search routines and a minor bugfix.


Release Notes: This release once again restores compatibility with the Ensembl database, adds a button to view the alternative transcripts when retrieving a gene from Ensembl, updates the list of Ensembl genome databases, and adds several minor cosmetic enhancements to the GUI.


Release Notes: This release once again fixes compatibility with the Ensembl databases.


Release Notes: This release restores compatibility with the Ensembl databases and adds an indication of sequence length to the "View intron/exon structure" window for real-time PCR.


Release Notes: This release adds new options for the design of QPCR primers, display of GC% in primer information, an option to set the wordsize parameter for BLAST searches, and updated Ensembl databases. It also fixes several small bugs.


Release Notes: This release fixed a crash that occurred when [dNTPs] > [Mg++], correctly lists the oligo concentration in the preferences as being in nM, and includes a tutorial in the distribution.


Release Notes: This release included the ability to use a local BLAST server instead of the NCBI Web server for BLAST searches, automatic calculation of full primer-dimer stability for primer pairs, and a view intron/exon structure function for Real-time PCR.


Release Notes: This release fixes a number of small bugs concerning opening sequences and the display of Bisulphite and Sequencing primers.


Release Notes: This release fixed a potentially serious bug when adding cloning sequences, in which the restriction enzyme site and anchor sequence for the reverse primer were swapped in order.